Effect of Ghrelin on nuclear factor-κB and tumor necrosis factor-α in the cerebral cortex of immature rats with pilocarpine-induced epilepsy
10.3760/cma.j.issn.1673-4912.2010.06.020
- VernacularTitle:Ghrelin对匹罗卡品诱导癫(癎)大鼠大脑皮层核因子-κB和肿瘤坏死因子-α表达的影响
- Author:
Ruiyun ZHANG
;
Qingyi WANG
;
Peiguo LI
;
Fengxuan SUI
;
Hua WANG
- Publication Type:Journal Article
- Keywords:
Epilepsy;
Cerebral cortex;
Pilocarpine;
Ghrelin;
rats
- From:
Chinese Pediatric Emergency Medicine
2010;17(6):532-534,后插2
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of gene and protein expressions of nuclear factor-κB(NF-κB) and tumor necrosis factor-α (TNF-α) in immature rats with pilocarpine-induced epilepsy treated with ghrelin. Methods The pllocarpine-induced epilepsy model in immatured rats were built, then the rats were divided into three groups: Ghrelin-treated group, saline-treated group and model group, meanwhile the normal control group was set. The NF-κB and TNF-α levels of gene and protein in the cerebral cortex of immature rats were detected. Results The expression levels of gene and protein of NF-κB and TNF-α were increased in model group,but decreased in the normal control group;NF-κB and TNF-α levels in Ghrelin treated group were obviously lower than those of saline-treated group and model group(P < 0. 05). Conclusion The protective mechanism of Ghrelin for nerve cell is cutting down the expressions of NF-κB and TNF-α in the cerebral cortex of immature rats with epilepsy and lessening inflammatory reaction in neurocytes.
