The detection of expression and significance of TGase3 mRNA in esophageal carcinoma by tissue microarray and in situ hybridization methods
- VernacularTitle:组织芯片原位杂交法检测TGase3 mRNA在食管癌中的表达及意义
- Author:
Li XUE
;
Jing WU
- Publication Type:Journal Article
- Keywords:
Transglutaminase 3mRNA;
Tissue microarray;
In situ hybridization;
Esophageal carcinoma
- From:
Cancer Research and Clinic
2007;19(z1):11-13,16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of TGase3 mRNA in esophageal carcinoma and to investigate it's role and the significance of in the carcinogenesis and progression of esophageal carcinoma.Methods The expression of TGase3 mRNA in 70 ca8es of esophageal carcinoma tissue and 10 cases of normal esophageal tissue were detected by tissue miemarray(TMA)and in situ hybridization(ISH)methods.Results The loss expression rate of TGase3mRNA in esophageal carcinoma tissue was 68.57%(48/70),which was significantly higher than in normal control(P＜0.05).The expression of TGase3 mRNA was no correlation with patients'sex,age,tumor site,lymph node metastasis.But it's significant correlation with clinical stage,histology grade,Pathology grade(P＜0.05).Conclusion There was a high frequency of TGase3 mRNA expression loss in esophageal carcinoma,TGase3 mRNA gene may be involved in carcinogenesis and progression of esophageal carcinoma.
