The tyrosine phosphatase SHP-2 participated in the cell migration induced by IL-1β
- VernacularTitle:蛋白酪氨酸磷酸酶SHP-2参与白细胞介素-1β促发的细胞移动过程
- Author:
Fengmei WANG
;
Zhichao FU
;
Jing XU
- Publication Type:Journal Article
- Keywords:
Protein-tyrosine phosphatase;
Cadherin;
Matrix metalloproteinase 9;
Interleukin-1 beta
- From:
Cancer Research and Clinic
2008;20(12):796-798,808
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the tyrosine phosphatase SHP-2 participate in the cell migration induced by IL-1β.Methods Two different reorganized plasmids transfected into the MCF-7,and achieved two different cell lines:SHP-2-GFP-MCF-7 cell lines.SHP-2C＞S-GFP-MCF-7 cell lines.The cell migration was detected by a fluorescence microscope and the expression of E-eadhefin and MMP-9 wee detected by western blotting analysis and RT-PCR.Results The SHP-2-GFP-MCF-7 cell lines showed a higher cell migration ability,and the expression of E-cadherin in the SHP-2-GFP-MCF-7 cell lines was especially lower,neady undetectable.But in this group the expression of MMP-9 was the highest.Conclusion The tyrosine phosphatase SHP-2 participate in the cell migration induced by IL-1β,and maybe produce a marked effect by reinforcing the expression of MMP-9 and reducing the expression of E-cadherin.
