Schwann cells induced neuronal differentiation of adipose-derived stem cells (ADSCs)

VernacularTitle:许旺细胞诱导下脂肪来源干细胞向神经细胞方向分化的研究
Author: Boxiu LI ; Biao CHENG ; Cheng ZHANG ; Xiaojun CHU ; Zhengrong CHEN
Publication Type:Journal Article
Keywords: Schwann cell; Adipose-derived stem cells; Neural differentiation
From: Chinese Journal of Microsurgery 2009;32(2):119-122,illust 5
CountryChina
Language:Chinese
Abstract: Objective To investigate the function of SD rats Schwann cells on the growth and differentiation of adipose-derived stem cells(ADSCs) when they are co-cultured in Transwell culture system. Methods The ADSCs harvested from SD rats were divided into 3 groups. Group A: ADSCs and Schwann cells were cultured in Transwell indirect co-culture system. Group B: ADSCs were induced by β-BME and BHA. Group C: a contrast group. The morphology of ADSCs was observed and immunohistology was pedormed. Results ADSCs of group A and group B were partly differentiated to the cells which had long neurite,and had a positive result in the NF staining, but negative in the GFAP staining. Group A shows no great difference in neurite length compared with group B(P < 0.05), and no great difference in cell quantity compared with group C (P < 0.05). Conclusion Schwann cells of SD rat have a growth supportive and neuronal differentiation inducing effect on ADSCs.