Effect of ASODN transfection targeting VEGF-C gene on proliferation and apoptosis of Hela cells
10.3760/cma.j.issn.1006-9801.2009.07.002
- VernacularTitle:转染血管内皮生长因子C反义寡核苷酸对子宫颈癌Hela细胞增生和凋亡的影响
- Author:
Li QI
;
Lina XING
- Publication Type:Journal Article
- Keywords:
Uterine cervical neoplasms;
Hela cells;
Vascular endothelial growth factor C;
Oligonucleotides,antisense;
Transfection;
Apoptosis
- From:
Cancer Research and Clinic
2009;21(7):437-439,443
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore effect on the proliferation and apoptosis of Hela cells in vitro by using vascular endothelial growth factor-C(VEGF-C) antisense oligonucleotides (ASODN). Methods VEGF-C ASODN was transfected into Hela cells by liposome-mediated; the cells transfected with the oligodeoxynuclecotide (SODN) and saline were used as control groups. The efficiency of transfection was detected by fluorescence microscope. The inhibitive rate of cell growth was detected with MTT methods. Apoptosis and cell cycle were evaluated using FCM. The mRNA expression of VEGF-C was determined by RT-PCR, and the expression of VEGF-C was determined by western blotting. Results VEGF-C ASODN had been transfected into Hela cells by liposome-mediated. The index of apoptosis after transfection 48 h was (19.39±1.81)%. G2/M phase cell increased after transfection, meanwhile the expression of VEGF-C degraded on the level of mRNA and protein (P<0.05). Conclusion Transfeeted with VEGF-C ASODN could down-regulate the expression of VEGF on the level of mRNA and protein, block the cell circle, inhibit cell proliferation and induce apoptosis in Hela cells in vitro.
