Effect on CYP1B1 expression of transplanted-A2780TS cells in nude mice paclitaxel resistance ovarian cancer by α-NF

VernacularTitle:α-奈黄酮对人类卵巢癌紫杉醇耐药细胞裸鼠移植瘤CYP1B1基因表达的影响
Author: Zhuangyan ZHU ; Yaqin MU ; Xiaomin FU ; Fuxi ZHAO
Publication Type:Journal Article
Keywords: Ovarian neoplasms; α-NF; Gene,CYP1B1; Paclitaxel; Drug resistance,neoplasm
From: Cancer Research and Clinic 2009;21(8):441-443,446
CountryChina
Language:Chinese
Abstract: Objective To study the effects of flavone compound α-NF on CYP1B1 gene in transplanted nude mice tumor with human paclitaxel resistance ovarian cancer cell. Methods Using 24 nude models of orthotopic transplantation of human ovarian cancer, they were divided randomly into four groups: control group, α-NF group, paclitaxel group and combined group. RT-PCR and Western blotting method were used to detect CYP1B1 mRNA and protein expression. Results There was no significant difference in CYP1B1 mRNA expression between α-NF group, combined group and control group(P >0.05). Compared with control group, CYP1B1 protein expression was distinctly lower in α-NF and combined group (P<0.01). Conclusion α-NF has no effect on CYPIB1 gene in mRNA level, whereas α-NF down-regnlate, CYP1B1 expression of transplanted A2780TS nude mice tumor in protein level, thus α-NF has the effect on reversing paclitaxel resistance of A2780TS cell.