Effects of erythropoietin on Schwann cells in vitro
10.3760/cma.j.issn.1001-2036.2009.05.013
- VernacularTitle:红细胞生成素对体外培养许旺细胞的作用
- Author:
Xuejian WU
;
Shengyang DU
- Publication Type:Journal Article
- Keywords:
Erythropoietin;
Schwann cells;
Peripheral nerve
- From:
Chinese Journal of Microsurgery
2009;32(5):381-383,illust 4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of EPO on GDNF secretion and cell cycle of Schwann cells(SCs) in vitro,and explore how EPO improve peripheral nerve regeneration. Methods Devided the purified Schwann cells of primary culture into three groups:group A with DMEM culture solution contained 10%fetal bovine serum and without EPO,group B with DMEM culture solution as above and 10 U/ml EPO,group C with DMEM culture solution as above and 50 U/ml EPO.The GDNF level of each group was detected in the culture by ELISA,and carried out the flow cytometry of Schwann cells on each group.Results GDNF in culture solution of group B,group C was more than that of group A,and the S-stage rate(S%)and (S+G_2M)%of Schwann cells in group B and group C more than that of group A. Conclusion EPO can increase the GDNF secretion and enhance proliferation activity of Schwann cells,which can explain how EPO improve peripheral nerve regeneration.
