The evaluation of biological potential for construction of tissue-engineered bone by combination of oscillationg seeding with encapsulated BMP-2 gene
10.3760/cma.j.isan.1001-2036.2009.05.014
- VernacularTitle:凝胶包埋BMP-2基因及振荡构建工程化骨的生物效应评估
- Author:
Honggang GUO
- Publication Type:Journal Article
- Keywords:
Adipose derived stromal cells;
BMP-2;
Gene transfer;
Tissue engineering
- From:
Chinese Journal of Microsurgery
2009;32(5):384-386,illust 5
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the osteogenic potential of tissue-engineered bone in vitro,and to evaluate an effective way in constructing tissue-engineered bone.Methods Tissue-engineered bone was constructed in the following ways:ADSCs were transfected with BMP-2 gene plus static seeding and static culture (group A),ADSCs were encapsulated with gels containg BMP-2 plus oscillating seeding and static culture (group B),gels encapsulation with BMP-2 gene and oscillating seeding(group C),gels encapsulation with BMP-2 and static seeding(group D).SEM and CLM were used to observe cellular pattern,apoptosis rate,cell activity,ALP level were detected at 1,4,8,12,16,20,24,28 days.New bone formation were observed after transplantation of bone construct in nude mice. Results Under observation of SEM and CLM. new tissue showed more remarkable cell proliferation with abundant ECM in group C,compared with other groups,the apoptosis rate in group C was significantly lower(P＜0.05),and level of cell activity,ALP,DNA and OCN were significantly higher(P＜0.05). Conclusion Gels encapsulation of BMP-2 gene combined with oscillating seeding promotes the cell augmentation and improves the quality of tissue-engineered bone.
