Detection of EGFR gene mutations in patients with non-small cell lung cancer by denaturing high performance liquid chromatography technology

VernacularTitle:变性高效液相色谱技术检测非小细胞肺癌患者表皮生长因子受体基因突变的研究
Author: Dan CAI ; Yunbin YE ; Qiang CHEN ; Xiongwei ZHENG ; Ying SU ; Shuoyan LIU ; Jing JIA ; Chao LI ; Xiaoling HE ; Ling ZHONG
Publication Type:Journal Article
Keywords: Carcinoma,non-small cell lung; Gene,erbB-1; Mutation; Chromatography liquid
From: Cancer Research and Clinic 2010;22(9):595-597,600
CountryChina
Language:Chinese
Abstract: Objective To investigate the advantages of detection for EGFR gene mutations by denaturing high performance liquid chromatography (DHPLC) technology. Methods DHPLC was used to detect EGFR gene mutations at exon 19 and 21 in 49 cases of non-small cell lung cancer (NSCLC) patients,and the direct DNA sequencing was used to verify the accuracy of DHPLC detection. Results EGFR gene mutation was identified from 13 of 49 cases by DHPLC,including deletion mutation at exon 19 in 10 cases (76.92 %) and alternative mutations at exon 21 in 3 cases (23.08 %). Mutation results of DHPLC was consistent with DNA direct sequencing. The results of the direct DNA sequencing were the same as those of DHPLC. The sensitivity of mutation test by DHPLC was 100 %. Conclusion DHPLC technology can be used for large scale screening of EGFR gene mutation with rapid and accuracy.