MRI monitoring superparamagnetic iron oxide (SPIO) particles labeling Schwann cells in vitro
10.3760/cma.j.issn.1001-2036.2010.05.009
- VernacularTitle:超顺磁性纳米铁颗粒标记许旺细胞及体外磁共振示踪的实验研究
- Author:
Jinbao QING
;
Zunli SHEN
;
Jiaxue ZHU
;
Xiaopan WANG
;
Kangan LI
;
Yuqing JIN
- Publication Type:Journal Article
- Keywords:
Schwann cells;
Superparamagnetic iron oxide (SPIO);
Labeling in vitro;
MRI
- From:
Chinese Journal of Microsurgery
2010;33(5):381-383,后插6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of labeling Schwann cells with different concentrations of SPIO, and to investigate the feasibility of in vitro MR imaging. Methods The C57BL/6 mices'Schwann cells were isolated, purified, and then 0.5 × 106, 1.0 × 106, 5.0 × 106 cells were labeled with 25.0 μg/ml, 50.0 μg/ml SPIO. Prussian blue stain and transmission electron microscope (TEM) were performed for showing intracellular iron. The signal intensity of cells were evaluated by 3.0 MRI with different sequences in vitro. Results Different cell population (0.5 × 106, 1.0 × 106,5.0 × 106) were cultured with different concentration SPIO about 24 hours. Dyeing degree of labeling cells stained by Prussion blue gradually deepened from 25.0 μg/ml to 50.0 μg/ml. Transmission electron microscope indicated that iron particles accumulated inendosomes/lysosomes. The MR signal intensity of labeling cells were inversely correlated with the concentration of SPIO groups in T2WI and GRE/30° imaging in vitro. Conclusion Schwann cells could be labeled effectively with SPIO, and MRI could be used to monitor these labeled cells in vitro.
