Effect of activated Notch signaling system on Schwann cells
10.3760/cma.j.issn.1001-2036.2010.05.008
- VernacularTitle:激活的Notch信号系统对许旺细胞调控作用的影响
- Author:
Jin WANG
;
Lei DENG
;
Yanhua WANG
;
Peixun ZHANG
;
Hongbo ZHANG
;
Baoguo JIANG
- Publication Type:Journal Article
- Keywords:
Schwann cells;
Notch signaling system;
NGF
- From:
Chinese Journal of Microsurgery
2010;33(5):378-380,后插6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of activated Notch signaling system on Schwann cells in vitro. Methods Schwann cells were isolated from sciatic nerves of adult SD rats. Recombinant rat jagged1/FC chimera, an activator of the Notch signaling system, and γ-secretase inhibitor (DAPT), an inhibitor of the Notch signaling system, were added into the culture medium respectively. The cells in the medium added with phosphate buffered saline were used as control group. Then the cultured cells were collected. NICD, as a mark of activated Notch system, was detected by immunofluorescence methods. MTT method was used to calculate the growth curve. The level of NGF in the cell supernatant was assayed by using ELISA. Results Schwann cells in recombinant rat jagged/FC chimera group grew better than those in the control groups. MTT method showed that activated Notch signaling significantly promote the proliferation of the cells(P ＜ 0.05). After cultured for 48 h, the amount of NGF in cell supernatant of recombinant rat jagged1/FC chimera group was significantly increased (P ＜ 0.05). Conclusion When the Notch signaling system is activated, Schwann cells not only proliferate but also secret more NGF.
