The biological response of loading ADSCs to surface topography of biomimetic matrix combined PRP with RGD modification
10.3760/cma.j.issn.1001-2036.2010.06.011
- VernacularTitle:PRP及RGD联合修饰表面改性后仿生基质对ADSCs生物学行为的调控
- Author:
Honggang GUO
;
Jing LIU
;
Fanglian YAO
;
Fang WANG
;
Xiaomian LI
;
Xinlong MA
- Publication Type:Journal Article
- Keywords:
Adipose derived stromal cells;
Platelet rich plasma;
Arg-Gly-Asp;
Surface topography;
Biomimetic matrix
- From:
Chinese Journal of Microsurgery
2010;33(6):469-472,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the biological potential of surface topography of biomimetic matrix combined PRP gels with RGD modification. Methods Surface topography of nβ-TCP/Cs/PCL matrix was made by Nd: YAG laser, tissue-engineered bone was constructed in the following ways: ADSCs were loaded to nβ-TCP/Cs/PCL matrix with PRP gels plus RGD modification (group A), ADSCs were cultured to nβ-TCP/Cs/PCL matrix with RGD modification (group B), ADSCs implantation with topography-surfaced treatment of matrix (group C), ADSCs cultivation with smooth-surfaced treatment of matrix (group D). SEM and CLM were used to observe cellular pattern, survival rate, cell activity, ALP and collagen type Ⅰ level were detected at 1, 4, 8, 12, 16, 20, 24,28 days. Runx2 and OPG expressions were assayed at different interval. Results Under observation of SEM and CLM, new tissue showed more remarkable cell proliferation with abundant ECM in group A. Compared with other groups, the survival rate in group A was significantly higher (88.16 ± 1.29, P ＜ 0.05),and the level of cell activity, ALP, collagen type Ⅰ were significantly higher (0.92± 0.13, 87.27 ± 3.08, 93.27 ± 3.91, P＜ 0.05), and remarkable expression of Runx2 and OPG was also seen. Conclusion Topography-surfaced treatment of matrix combined PRP gels with RGD modification enhances the cell proliferation and acts as a feasible osteopromotive method.
