Advances in Molecular Modification of Microbial Enzymes
- VernacularTitle:微生物酶分子改造研究进展
- Author:
Zhaowei GAO
;
Yuhong ZHANG
;
Wei ZHANG
- Publication Type:Journal Article
- Keywords:
Enzyme Molecular modification Directed evolution Gene engineering
- From:
China Biotechnology
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
In recent years,more and more of the enzyme proteins have been carried out using recombinant microorganism bioreactor for large scale production.For reasons of improved the catalytic capability and environmental suitability,or enhanced expression level of the protein,a variety of genetic engineering technology according to protein molecule modification have been applied extensively.Major strategies and achievements of molecular modification for microbial enzyme,such as site-directed mutagenesis,error-prone PCR,DNA shuffling and optimum codon design were reviewed.
