The SPIO labeling of mature dogs BMSCs in vitro

VernacularTitle:成年犬骨髓间充质干细胞的体外SPIO标记研究
Author: Ying CHEN ; Liming CHENG ; Zirong LI ; Zhongshi LI ; Zhe CAI ; Lin PAN
Publication Type:Journal Article
Keywords: Bone marrow stromal cells; Superparamagnetic iron oxide; Label in vitro; Magnetic resonance imaging
From: Chinese Journal of Microsurgery 2010;33(6):457-460,后插6
CountryChina
Language:Chinese
Abstract: Objective To explore labeling efficiency and appropriate conditions of Superpara magnetic iron exide nanopaticles (SPIO) nanoparticles for Bone marrow stromal cells(BMSCs). Methods BMSCs were aquired from skeletally mature dogs via iliac crest aspiration and separated by adherent cell cytopheresis.BMSCs were cultured and incubated with SPIO at different concentrations in vitro. The labeling efficiency of BMSCs with different labeling concentrations SPIO nanoparticles as well as detection of characteristics and signal attenuation rules were evaluated by MRI at 1.5T in vitro. Results BMSCs were efficiently labeled by SPIOin vitro and has no alterations to viability and proliferation profiles at this labeling concentration. BMSCs loaded with SPIO can be detected by MRI at certainly cell quantity in vitro(5 × 104). The quantity of SPIO in cells gradually reduced as cell culture time prolonged, with no statistically significant changes in cell death(P＞ 0.05). Conclusion The results demonstrated the potential application of SPIO as a wonderful cell tracer in vitro.