High performance liquid chromatography for determining the isofraxidin content in Feitai Capsule
- VernacularTitle:高效液相色谱法测定肺泰胶囊异秦皮啶含量
- Author:
Zongyang YU
;
Xuenong OUYANG
;
Quanshan HE
;
Xihu DAI
;
Guixiang ZHONG
;
Jia YAN
;
Zhizhen LIU
- Publication Type:Journal Article
- Keywords:
Feitai Capsule;
High performance liquid chromatography;
Isofraxidin;
Determination
- From:
Journal of Medical Postgraduates
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Feitai Capsule,a compound of traditional Chinese herbal medicine,has been screened and refined repeatedly for many years and shown to have a good anti-tumor effect.Strict quality control and further screening of the efficacious components of the compound are of great clinical significance.The purpose of this study was to establish the methods for determining the isofraxidin content in Feitai Capsule.Methods We determined the content of isofraxidin in Feitai Capsule by high performance liquid chromatography (HPLC),using the chromatographic column Hypersil ODS-C18 (4.6 mm?150 mm,5 ?m),with the mobile phase as acetonitrile 0.2% phosphoric acid solution (21∶79),the flow rate of 1.0 ml/min,the detective wavelength of 344 nm and the column temperature at 30℃.Results Isofraxidin showed a good linearity,within the range of 2.00-10.80 ?g/ml (y=69 427x+15961,r = 0.999 9),with the average recovery of 97.89% and RSD of 1.64% (n = 6).Conclusion HPLC,accurate and reproducible,is suitable for the determination of the isofraxidin content in Feitai Capsule.
