Acute application of ApoE4 increases the neuronal resting[Ca~(2+)]i in rat cortex
- VernacularTitle:急性给予ApoE4升高大鼠皮层神经元内静息[Ca~(2+)]i
- Author:
Xinai LIU
;
Xinyi LI
- Publication Type:Journal Article
- Keywords:
ApoE;
intracellular calcium;
NMDA;
Fluo-3/AM
- From:
Basic & Clinical Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the acute effect of apolipoprotein E(apoE)on the intracellular free Ca2+ of rat cortical neurons.Methods The intracellular resting calcium level in cultured primary rat cortical neurons was measured by using confocal fluorescent imaging technique. MK-801,an N-methyl-D-aspartate (NMDA) receptor noncompetitive antagonist,was employed to test potential function of apoE4 through blocking NMDN receptor. ResultsAcute application of apoE4,but not apoE3,significantly increased the resting [Ca2+]i in a dose-and time-dependent manner (P
