NDRG2 gene transfection inhibits proliferation of breast cancer cell
- VernacularTitle:转染NDRG2基因抑制乳腺癌细胞增殖
- Author:
Cailin ZHU
;
Nanlin LI
;
Ting WANG
- Publication Type:Journal Article
- Keywords:
NDRG2;
tumor;
gene transfection;
adenovirus
- From:
Basic & Clinical Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of gene NDRG2(N-myc downstream regulated gene 2) transfection on the proliferation and apoptosis of breast cancer cell.Methods Bcap37 cell line expressing low level product encoded by NDRG2 gene is transfected with recombinating adenovirus expressing high level NDRG2 gene product to improve NDRG2 gene expression level. NDRG2 expression was detected by Western blot and the proliferation activity of the cell lines was detected by MTT and flow cytometry. Results High level expression of NDRG2 inhibits cells growth and results in G1 phase arrest in Bcap37 cell line. Compared with non-transfected Bcap37 cells and Bcap37 cells transfected by empty vector,apoptosis cells obviously increase in Bcap37 cell line transfected by recombinating adenovirus expressing high level NDRG2 gene,12% after 48 hours and 21.5% after 72 hours. Conclusion Overexpression of NDRG2 in Bcap37 cells effectively inhibites cell proliferation and induces cell cycle arrest and apoptosis.
