Effect of gross saponins of Tribulus terrestris on cardiocytes impaired by adriamycin
- VernacularTitle:蒺藜皂苷对阿霉素损伤心肌细胞的保护作用
- Author:
Shuang ZHANG
;
Hong LI
;
Hui XU
;
Shijie YANG
- Publication Type:Journal Article
- Keywords:
gross saponins of Tribulus terrestris;
cardiocyte;
adriamycin;
apoptosis;
oxygen free radical
- From:
Acta Pharmaceutica Sinica
2010;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
This study is to observe the protection of gross saponins of Tribulus terrestris (GSTT) on cardiocytes impaired by adriamycin (ADR) and approach its mechanism of action. Cardiocytes of neonate rat were cultivated for 72 hours and divided into normal control group, model (ADR 2 mg?L-1) group, and GSTT (100, 30, and 10 mg?L-1) groups. MTT colorimetric method was deployed to detect cardiocyte survival rate, activities of CK, LDH, AST, SOD, MDA and NO were detected, and apoptosis was detected with flow cytometry. Effect of GSTT on caspase-3 was detected with Western blotting. Compared with control group, contents of CK, LDH, AST, MDA and NO were increased, and activity of SOD was reduced (P
