Effect of m-nisoldipine on the Ca~(2+)/CaM/CaN signal pathway in 5-HT-induced proliferation of rat PASMCs
- VernacularTitle:间尼索地平对5-羟色胺诱导的大鼠肺动脉平滑肌细胞增殖中Ca~(2+)/CaM/CaN通路的影响
- Author:
Xueyan CHEN
;
Huanlong LIU
;
Zhenhua PAN
;
Qingfeng MIAO
;
Yongjian ZHANG
- Publication Type:Journal Article
- Keywords:
m-nisoldipine;
5-hydroxytryptamine;
PASMCs;
proliferation;
Ca2+
- From:
Acta Pharmaceutica Sinica
2010;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
This study is to explore the activation of the Ca2+/CaM/CaN signal pathway in 5-HT-induced proliferation of rat pulmonary artery smooth muscle cells (PASMCs) and the inhibitory effect of m-nisoldipine (m-Nis) on this pathway. PASMCs were cultured with the explant technique. The proliferation of PASMCs was evaluated by MTT assay. Confocal microscopy was used to measure the change of [Ca2+]i. The mRNA expression of CaM and CaN was evaluated by RT-PCR and the activity of CaN was measured according to the instruction of kits. The results of MTT assay suggested that 5-HT (1 ?mol?L-1) significantly induced the proliferation of rat PASMCs (P
