Cloxacillin-potentiated Disc Diffusion Test to Detect AmpC ?-Lactamases in Gram-negative Bacilli Strains
- VernacularTitle:氯唑西林增效试验对肠杆菌科高产AmpC酶细菌的表型检测
- Author:
Zihe YAN
;
Xichi HU
;
Feng WU
- Publication Type:Journal Article
- Keywords:
AmpC ?-lactamases;
Cloxacillin-potentiated disc diffusion test;
Cefoxitin three-dimesional test
- From:
Chinese Journal of Nosocomiology
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To develop a suitable and easy method for detecting AmpC ?-lactamases.METHODS The function with different cloxacillin concentrations in cloxacillin-potentiated disc diffusion test was compared and estimated with FOX three-dimensional extract test to show the best concentration of cloxacillin.RESULTS Cefoxitin three-dimesional test indicated that 48 strains were detected out to produce AmpC ?-lactamases among 130 clinical isolates of Gram-negative bacilli strains.when the discs contained 200 ?g cloxacillin the CAZ,CTX,CRO and ATM alone could separately detect 87.5%,87.5%,87.5% and 33.3% highly producing AmpC ?-lactamases strains.When the combination of CTX with CRO,almost 95.8% could be detected out.CONCLUSIONS With the combination of CTX and CRO,the cloxacillin-potentiated disc diffusion test can specifically detect AmpC ?-lactamases and easy to be used in clinical laboratories.
