Application of a Simple Method for Detection of Rubella Virus Genome by Loop-mediated Isothermal Amplification (LAMP)
- VernacularTitle:应用简便的逆转录环介导等温扩增方法检测风疹病毒核酸
- Author:
Shuang WANG
;
Jianhui ZHOU
;
Xiang HOU
- Publication Type:Journal Article
- Keywords:
Rubella virus;
Reverse transcription loop-mediated isothermal amplification;
Nucleic acid
- From:
Chinese Journal of Vaccines and Immunization
2008;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To use a new simple Reverse Transcription Loop-mediated isothermal amplification(RTLAMP) method was applied to detect rubella virus nucleic acid and compared with Reverse TranscriptionPolymerase Chain Reaction (RT-PCR).Method Comparing the detection rate of the RT-LAMP method with that of RT-PCR for detecting rubella virus nucleic acid from rubella virus.Results The nucleic acid positive rates of all 11 strains of rubella virus were 100% by the two methods,the positive rate was 55%.Conclusion RT-LAMP is more simple and convenient than RT-PCR.
