Effect mechanism of neuroprotection of Sinomenine on rats with cerebral ischemia-reperfusion
- VernacularTitle:青藤碱对缺血再灌注大鼠脑保护的作用机制
- Author:
Hao LI
;
Lan WU
;
Suxian ZHOU
- Publication Type:Journal Article
- Keywords:
Sinomenine;
ischemia-reperfusion;
nuclear factor-?Bp 65;
intercellular adhesion molecule-1;
myeloperoxidase
- From:
Journal of Clinical Neurology
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect mechanism of neuroprotection of Sinomenine(Sin) on rats with cerebral ischemia-reperfusion(IR).Methods 80 Wistar rats were randomly divided into sham-operated group,IR group,Sin high-dose(60 mg/kg)group and Sin low-dose(30 mg/kg)group.The correlative dose of Sin were intraperitoneal injected in Sin high-dose and low-dose groups,respectively.30 min later,the IR models were made.After 24 h of IR,the volume of cerebral infarction(CI) and the change of cerebral pathology were observed by TTC and HE staining.The brain water content was investigated by dry and wet weight method.The expression of nuclear factor (NF)-?Bp65,intercellular adhesion molecule (ICAM)-1 and the activity of myeloperoxidase (MPO) in the frontal and parietal cortex were detected by immunohistochemistry.Results Compared with IR group,the ischemic impairment in Sin high and low-dose groups were obviously lighter,even lighter in Sin high-dose group;the CI volumes and brain water content were significantly reduced;while,even lower in Sin high-dose group(all P
