Change of hippocampal neurons cholinergic receptor in rats with vascular dementia
- VernacularTitle:血管性痴呆大鼠海马神经型胆碱能受体的变化
- Author:
Yan XIAO
;
Changxue WU
;
Zhizhong GUAN
- Publication Type:Journal Article
- Keywords:
vascular dementia;
acetylcholinesterase;
nicotinic acetylcholine receptor
- From:
Journal of Clinical Neurology
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the change of hippocampal neurons cholinergic receptor in rats with vascular dementia(VD).Methods VD rat models were established by employing improved method of Pulsinelli's four-vessel occlusion.1 month later,the abilities of learning and memory of VD models were tested by Morris water maze.The activities of acetylcholinesterase (AChE) in plasma and hippocampus were detected by the improved Ellman's colorimetric methods.The expressions of hippocampal nicotinic acetylcholine receptor (nAChR) subunits ?3,?4 and ?7 proteins and mRNA were detected respectinely by Western Blotting and RT-PCR,respectively.The results were compared with sham operation group.Results Compared to the sham operation group,the escape latency of Morris water maze test and the first time crossing platform in VD group were significantly prolonged,the number of crossing platform was decreased (all P
