Typing of Pandrug-resistant Acinetobacter baumannii in Hospital by Randomly Amplified Polymorphic DNA
- VernacularTitle:全耐药鲍氏不动杆菌的随机扩增DNA多态性分析
- Author:
Aiping YANG
;
Jinhong YANG
;
Fangqu LI
;
Xiangyang LI
- Publication Type:Journal Article
- Keywords:
Pandrug-resistant Acinetobacter baumannii;
Randomly amplified polymorphic DNA technique;
Genotyping;
Nosocomial infections
- From:
Chinese Journal of Nosocomiology
2009;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the homology of the pandrug-resistant Acinetobactor baumannii(PRABA) by randomly amplificed polymorphic DNA(CRPD) to guide the control of the nosocomial infection and epidemology investigation.METHODS Eighteen strains of PRABA were collected from Jul 2008 to Mar 2009 in our hospital.The bacteria were examined by DADE BEHRING Microscan WalkAway 96SI;DNA extracted from all isolates was amplified by polymerase chain reaction using random primers.In addition epidemology investigation and antimicrobial susceptibility testing were also under taken.RESULTS Fifteen strains were divided into 6 RAPD profiles.There were two clonal strains derived,respectively,from two intensive care units.One strain was isolated from a patient in surgical ward who was transferred from ICU1,showing same homology with that in the ICU1.The homology was different from sensitive strains and drug-resistant strains obviously.CONCLUSIONS An outbreak of PRABA happens in intensive care unit.
