Thiry-nine Types of Resistant-related Genes in a Pan-resistant Alcaligenes xylosoxidans subsp xylosoxidans in Sputum from a Severe Chronic Hepatitis Patient
- VernacularTitle:慢性重型肝炎患者痰泛耐药木糖氧化产碱菌木糖氧化亚种39种耐药基因的研究
- Author:
Zhijun SU
;
Desong MING
- Publication Type:Journal Article
- Keywords:
Severe hepatitis;
Pan-resistance;
Alcaligenes xylosoxidans subsp xylosoxidans;
?-Lactamases;
Aminoglycoside-modifying enzymes;
Chlorhexidine-sulfadiazine resistant gene;
Integron
- From:
Chinese Journal of Nosocomiology
2009;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study 39 kinds of resistant-related genes in a pan-resistant Alcaligenes xylosoxidans subsp xylosoxidans(AXXxx) in the sputum isolated from a severe hepatitis B patient.METHODS The susceptibility to antimicrobial agents were detected by MIC.16S rRNA and 39 resistant-related genes including 29 ?-lactamases genes,6 aminoglycoside-modifying enzymes(AMEs)genes,1 chlorhexidine-sulfadiazine resistant gene(qacE△1-sul1)and 3 intergron genes separated(intⅠ1,2,3) an AXXxx strain in the sputum of a severe hepatitis B patient were measured by PCR,and verified by DNA sequencing.RESULTS Among the strains,7 kinds of resistant-related genes(blaTEM-116,blaCARB-8,aac(6′)-Ⅱ,aac(3)-Ⅱ,ant(3″)-Ⅰ,qacE△1-sul1,and intI1)detected out.But other 27 kinds of ?-lactamases genes,3 kinds of AMEs(aac(6′)-Ⅰb,aac(3)-Ⅰ,ant(2″)-Ⅰ) genes,and 2 kinds of intⅠ(intⅠ2 and intⅠ3) genes were negative.CONCLUSIONS The pan-resistant A.xylosoxidans,mainly relates to 7 kinds of resistant-related genes(blaTEM-116,blaCARB-8,aac(6′)-Ⅱ,aac(3)-Ⅱ,ant(3″)-Ⅰ,qacE△1-sul1,and intⅠ1).
