Aspirin inhibits the proliferation of rat cardiac fibroblasts induced by endothelin-1
- VernacularTitle:阿司匹林抑制内皮素-1诱导的大鼠心脏成纤维细胞增殖
- Author:
Zhiguo ZHANG
;
Li YANG
;
Yaping ZHENG
;
Yanfeng ZHAO
- Publication Type:Journal Article
- Keywords:
endothelin-1;
cardiac fibroblasts;
aspirin;
bradykinin;
nitric oxide
- From:
Basic & Clinical Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of aspirin on endothelin-1 induced proliferation of cardiac fibroblasts(CFs).Methods Isolated and cultured CFs from neonatal Sprague-Dawley rats(SD) were randomly divided into 7 groups: control,ET-1,aspirin,ET-1+aspirin 1 mmol/L,ET-1+aspirin 2 mmol/L,ET-1+aspirin 5 mmol/L and ET-1+aspirin1 10 mmol/L.CFs were counted by MTT assay.Cell cycle distribution was determined with a flow cytometer(FCM).-Proline incorporation was evaluated by scintillation counting.Nitric oxide(NO) was measured by colorimetry.Results 10-7mol/L ET-1 significantly increased the number of CFs and -Pro incorporation and decreased NO2-/NO3-secretion compared with the control group(P
