Expression and significance of PDGF-D and VEGF in gastric carcinoma
- VernacularTitle:PDGF-D和VEGF在胃癌中的表达及其意义
- Author:
Lin ZHAO
;
Caiquan ZHANG
;
Gang LIAO
;
Jiegen LONG
;
Weixue TANG
- Publication Type:Journal Article
- Keywords:
gastric carcinoma;
PDGF-D;
VEGF;
immunohistochemistry;
RT-PCR
- From:
Basic & Clinical Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of platelet-derived growth factor-D(PDGF-D) and bascular endothelial growth factor(VEGF) in gastric carcinoma and elucidate the potential relationship between their overexpression and clinical pathological characteristics.Methods Immunohistochemical assay was performed to detect the protein expression of PDGF-D and VEGF in tissues.Semiquantitative reverse transcription polymerase chain reaction(RT-PCR) was performed to evaluate the mRNA expression of PDGF-D and VEGF in part of random selecting gastric carcinoma samples.The correlation between the expression of PDGF-D and VEGF,and the relationship between the expression of PDGF-D,VEGF and the clinical pathological characteristics were analyzed.Results The protein expression of PDGF-D and VEGF in gastric carcinoma tissues were significantly higher than that in adjacent tissues and normal gastric mucosa(P
