Valproic acid inhibits the cardiomyocyte hypertrophy induced by angiotensin Ⅱ in rats
- VernacularTitle:丙戊酸抑制血管紧张素Ⅱ致大鼠心肌细胞肥大
- Author:
Ying LU
;
Shuang YANG
;
Wenjuan DU
- Publication Type:Journal Article
- Keywords:
valproic acid;
angiotensin Ⅱ;
cardiomyocyte;
hypertrophy
- From:
Basic & Clinical Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the inhibitory role of valproic acid(VPA) in hypertrophic cardiomycytes induced by angiotensin Ⅱ(AngⅡ)in rats.Methods The cultured rat cardiomyocytes were divided randomly into control group,hypertrophic group and VPA group.They were treated with AngⅡ to induce hypertrophy and then given with VPA.The morphologic changes of cardiomyocytes were observed under the contrast phase microscope and electron microscope.The mRNA levels of ?-MHC were exmained by reverse transcriptase polymerase chain reaction(RT-PCR) method.The protein expression of C-FOS was exmanined by immunohistochemistry method.ResultsStimulated by AngⅡ,the cardiomyocytes were enlarged under the contrast phase microscope and the ultrastructure also changed.After stimulated by AngⅡ,the mRNA level of ?-MHC and the protein expression of C-FOS increased in the hypertrophic cardiocytes.Given by VPA,these data decreased accordingly.Conclusion VPA may inhibit the cardiomyocyte hypertrophy induced by angiotensin Ⅱ in some degree.
