Forty Types of Resistant-related Genes in a Pan-resistant Pseudomonas aeruginosa
- VernacularTitle:全耐铜绿假单胞菌40种耐药相关基因的研究
- Author:
Desong MING
;
Jianliang ZHUANG
;
Zhijun SU
;
Zhishan ZHANG
;
Zunjin XIE
- Publication Type:Journal Article
- Keywords:
Pan-resistant bacteria;
Pseudomonas aeruginosa;
?-Lactamases;
Aminoglycoside-modifying enzymes;
Chlorhexidine-sulfadiazine resistant gene;
oprD2;
Integron
- From:
Chinese Journal of Nosocomiology
2009;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study 40 kinds of resistant-related genes in a pan-resistant Pseudomonas aeruginosa.METHODS To detect the susceptibility of antimicrobial agents by MIC,40 resistant-related genes including 29 ?-lactamases genes,porin oprD2 genes,6 aminoglycoside-modifying enzymes(AMEs)genes,chlorhexidine/sulfadiazine resistant gene(qacE△1-sul1)and intergron(intⅠ1,2,3),etc,form 1 strain of P.aeruginosa were measured by PCR,and verified by DNA sequencing.RESULTS In the strain,there were positive of 6 kinds of resistant-related genes(blaTEM,blaOXA10,aac(6′)-Ⅱ,aac(3)-Ⅱ,qacE△1-sul1 and intⅠ1),but without oprD2 genes.Twenty-seven kinds of ?-lactamases genes,4 kinds of AMEs(aac(6′)-Ⅰb,aac(3)-Ⅰ,ant(3″)-Ⅰ and ant(2″)-Ⅰ),and 2 kinds of intⅠ(intⅠ2 and intⅠ3) were negative.CONCLUSIONS The multi-resistant mechanisms of pan-resistant P.aeruginosa are mainly related to 7 kinds of resistant-related genes(blaTEM,blaOXA10,oprD2,aac(6′)-Ⅱ,aac(3)-Ⅱ,qacE△1-sul1 and intⅠ1).
