Clinical Application of D-test in Pseudomonas aeruginosa
- VernacularTitle:铜绿假单胞菌中D-试验在临床中的应用
- Author:
Meijie JIANG
;
Xufeng MA
;
Huiqing SUN
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Imipenem;
Ceftazidime;
D-test;
Incidence rate
- From:
Chinese Journal of Nosocomiology
2009;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the incidence rate of Pseudomonas aeruginosa whose ceftazidime resistance was induced by imipenem and the relationship of excretion pump with sensibility of imipenem and ceftazidime.METHODS The sensibility to 15 antibacterials and ceftazidime resistance induced by imipenem were detected by slip diffusion method,detect these bacteriumas′s MIC to Imipenem and Ceftazidime before and after excretion pump inhibitor hydroxide radical cyanogen chlorine phenylhydrazone(CCCP) were added by agar dilution,detect Ceftazidime′s MIC value which were added different concentration imipenem by agar dilution.RESULTS From 325 strains 116 strains(35.7%) were imipenem induced ceftazidime-resistant drug fast,from them 80.2% were imipenem-resistant and ceftazidime-sensitive,19.8% were imipenem and ceftazidime both sensitive.Sensibility to imipenem and ceftazidime was no marked change before and after CCCP added,MIC value of ceftazidime was step up 4-12 times by imipenem.CONCLUSIONS The incidence rate of ceftazidime-resistant P.aeruginosa induced by imipenem is high,clinical microbiological laboratory should evolve D-test to guide clinician use antibacterials more reasonable.
