Inhibitory effect of octreotide on prolieration of rat hepatic stellate cells
- VernacularTitle:奥曲肽对大鼠肝星状细胞增殖的抑制作用
- Author:
Yongmei DAI
;
Limian CAI
;
Ling LIN
- Publication Type:Journal Article
- Keywords:
octreotide;
liver fibrosis;
hepatic stellate cells;
proliferation;
collagen;
matrix matrismetlloproteinases 2
- From:
Journal of Jilin University(Medicine Edition)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of octreotide (OCT) on gene and protein expressions of collagen Ⅰ,collagen Ⅲ and matrix matrixmetlloproteinases 2 (MMP-2)in hepatic stellate cells(HSC) in vitro,and to clarify the mechanism of its anti-hepatic fibrosis.Methods A rat hepatic stellate cell line,rHSC-99,was treated with OCT.Cell proliferation was assessed by MTT colorimetric assay.The contents of collagen Ⅰ,Ⅲ were examined by ELISA;RT-PCR was used to detect mRNA expressions of MMP-2,collagen Ⅰ and collagen Ⅲ.Results Compared with control group,OCT significantly inhibited rHSC-99 proliferation in vitro (P
