Expression of FSH receptor in ovary tissue of rats with letrozole-induced polycystic ovary syndrome
- VernacularTitle:来曲唑诱导多囊卵巢综合征大鼠卵巢组织中FSH受体的表达及其意义
- Author:
Hongsheng GUO
;
Changxin AN
;
Dong CHEN
- Publication Type:Journal Article
- Keywords:
polycystic ovarian syndrome;
disease model,animal;
letrozole;
receptors,FSH
- From:
Journal of Jilin University(Medicine Edition)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of FSH receptor mRNA and protein in ovary tissue in rats with letrozole-induced polycystic ovary syndrome(PCOS),and to provide experimental data for the model application. Methods Forty rats were randomly divided into two groups(n=20),in PCOS model group letrozole was administered once daily during 21 d,and in control group without any treatment.The gonadal hormone concentrations in serum were determined by radioimmunoassay,the histologic changes in ovaries were observed by HE staining,the expression of FSH receptor gene in ovary tissue was detected by realtime -PCR,Western blotting and immunohistochemistry.Results Compared with control group,estradiol(E2) and progesterone in model group showed a considerable reduction(P0.05).Compared with control group,the ovaries from model group showed high incidence of subcapsular ovarian cyst and capsular thickening and decreased number of corpora lutea.The expressions of FSH receptor mRNA and protein were significantly higher in model group than those in control group(P
