Isolation and detection of CK17~+,CD44v5~+ and Bcrp1~+ cells in HeLa cell line of cervical carinoma
- VernacularTitle:宫颈癌HeLa细胞中Bcrp1~+、CK17~+及CD44v5~+表型细胞的检测与分离
- Author:
Songling ZHANG
;
Xiaoxia ZHANG
;
Xiaowei YU
;
Yulin LI
- Publication Type:Journal Article
- Keywords:
HeLa cells;
carcinoma,squamous cell;
CSC;
CK17;
CD44v5;
Bcrp1;
flow cytometry
- From:
Journal of Jilin University(Medicine Edition)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify and isolate the CK17+,CD44v5+ and Bcrp1+ cells in HeLa cells,and investigate the relationship between them and select cervical cancer stem cell markers.Methods Flow cytometry was used to identify and isolate the CK17+,CD44v5+ and Bcrp1+ cells in HeLa cells;after isolation,the expressions of CK17 and CD44v5 in Bcrp1+ and Bcrp1-phenotype HeLa cells were measured by immunocytochemistry.Results HeLa cells contained 11% Bcrp1+ cells,0.7% CK17+ cells and 0.1%CD44v5+ cells.Bcrp1+ HeLa cells contained CK17+ and CD44v5+ HeLa cells,but the Bcrp1-Hela cells did not(P
