Effect of Small Needle-Knife on Collagens Ⅰ and Ⅲ in Transplanted Human Skin Hypertrophic Scar Tissues in Nude Mice
- VernacularTitle:小针刀对移植于裸鼠的人皮肤增生性瘢痕组织中Ⅰ、Ⅲ型胶原蛋白的影响
- Author:
Feng LI
;
Dongming CHEN
;
Jinlin QIAO
- Publication Type:Journal Article
- Keywords:
Small Needle-knife;
Nude Mice;
Hypertrophic Scars;
Collagen Fibre
- From:
Chinese Journal of Minimally Invasive Surgery
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the effect of small needle knife therapy on collagensⅠ and Ⅲ in the hypertrophic scar tissues that was subcutaneously transplanted into nude mice. Methods Six samples of human hypertrophic scar tissues without cuticle were subcutaneously transplanted into the back of 24 nude mice to create the animal models of hypertrophic scar. Ten days after of the operation,the mice were divided into control,0.1 mg/ml triamcinolone,0.2 mg/ml triamcinolone,and small needle-knife groups with 6 mice in each. Specimens of the scar tissues were collected in 14 days for immunohistochemistry to detect the collagen Ⅰ and Ⅲ. Results In all the groups,collagens Ⅰand Ⅲ distributed extensively in the cytoplasm of fibroblast and tissues. Image analysis showed that the concentration of collagens Ⅰand Ⅲ in 0.1 mg/ml and 0.2 mg/ml triamcinolone groups were significantly lower than those in the control (0.09?0.03,0.11?0.05 and 0.12?0.02,0.11?0.01 vs. 0.17?0.04,0.19?0.03,P0.05). Conclusions Small needle-knife therapy can reduce the concentration of collagens Ⅰand Ⅲ in transplanted hypertrophic scar tissues in nude mice.
