Western blot of the expression of J chain-human netrophil peptide-1
- VernacularTitle:人中性粒细胞防御素HNP1-J链融合多肽的免疫印迹检测
- Author:
Xianhua LIU
;
Xiaodong BAI
;
Jia CUI
- Publication Type:Journal Article
- Keywords:
Human neutrophil peptide;
Western blot;
Influencing factor
- From:
Journal of Medical Postgraduates
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Defensins from human neutrophils exhibit a broad antimicrobial activity.The transfection of the gene fused by human neutrophil peptide 1(HNP1) with the J chain into other cells may induce the in vitro expression of HNP1 to play antimicrobial roles.The authors analyzed the influencing factors on the expressed proteins of HNP1 and the J chain gene in COS-7 cells.Methods: Western blot was used to analyze the technical factors influencing the HNP1 protein expression.Results: Recombinant J-HNP1 was expressed in the cells successfully.Conclusion: The expression of J-HNP1 can be detected inside and outside the cells by Western blot.
