A Rapid Method by Type-specific Multiplex PCR for Clostridium perfringens on Surface of Traumatic Tissues
- VernacularTitle:型特异性多重PCR快速检测创伤组织中的产气荚膜梭菌
- Author:
Ying LIU
;
Huiying ZHANG
;
Yan WANG
;
Xiaoxia AN
;
Shuang QIU
- Publication Type:Journal Article
- Keywords:
Type-specific multiplex PCR;
Clostridium perfringens;
Trauma
- From:
Chinese Journal of Nosocomiology
2009;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a rapid detecting method for Clostridium perfringens on traumatic tissues by type-specific multiplex PCR.METHODS Established a simple and rapid method(TLS method) for purifying the DNA of genomes and plasmids in standard strains of C.perfringens wild strains on surface of open traumatic tissues and detected DNAs by type-specific multiplex PCR.RESULTS All types of C.perfringens could be detected by type-specific multiplex PCR.The sensitivity by PCR for type A of C.perfringens arrived at 7.5?103/ml and a whole run could finish within 5 h;the results by PCR entirely corresponded with those by cultureing.CONCLUSIONS New methods for purifying DNA of genomes and plasmids of C.perfringens are simple and rapid;there are high specificity and sensitivity for detecting DNA by multiplex PCR within short time,which can be practiced in clinical laboratory.
