Application of Polymerase Chain Reaction in Detection of Pathogens in Cerebrospinal Fluid
- VernacularTitle:聚合酶链反应在检测脑脊液病原菌中的应用
- Author:
Xiansen ZHANG
;
Changfa YU
;
Darong DUAN
;
Yingpeng REN
;
Ronghua RUAN
;
Xiaoming YING
- Publication Type:Journal Article
- Keywords:
Polymerase chain reaction;
Cerebrospinal fluid;
Pathogens
- From:
Chinese Journal of Nosocomiology
2009;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To set up a quick method to detect pathogens in cerebrospinal fluid.METHODS The method of polymerase chain reaction(PCR),using a pair of universal primers targeted at the 16S rRNA gene,was adopted to amplify the DNA of bacterium.138 clinical specimens obtained from patients were examined by PCR method and bacterial culture method.RESULTS The positive rate was 39.86% of PCR and 17.39% of culture,the outcomes had statistical significance(P
