Ligustrazine inhibited TNF-?-induced TGF-?1 and CTGF expression in human peritoneal mesothelial cells
- VernacularTitle:川芎嗪抑制TNF-?诱导的人腹膜间皮细胞TGF-?1和CTGF的表达
- Author:
Guisong ZHU
;
Jinsong HE
- Publication Type:Journal Article
- Keywords:
Ligustrazine;
tumor necrosis factor-?;
human peritoneal mesothelial cells;
transforming growth factor-?1;
connective tissue growth factor
- From:
Basic & Clinical Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Ligustrazine on TNF-?-induced TGF-?1 and CTGF expression in human peritoneal mesothelial cells (HPMCs). Methods HPMCs were isolated from human omenta by trypsin digestion. Then,the subcultured HPMCs were divided into control group,TNF-?-induced (1 ?g/L) group and TNF-?-induced plus low-,medium-and high-dose Ligustrazine (10,20 and 40 mg/L Ligustrazine respectively) groups. The viability of HPMCs was measured by MTT assay. RT-PCR was used to detect the expressions of TGF-?1 and CTGF mRNAs in HPMCs. TGF-?1 and CTGF in supernatants were measured by ELISA. Cell protein concentration was measured by trace bicinchoninic acid (BCA) method to validate the ELISA assay results.ResultsLigustrazine significantly decreases TNF-?-induced TGF-?1 and CTGF expression in a dose-dependent manner at both protein and gene levels (P
