Effects of human S100A6 on ?-catenin in two human osteosarcoma cell lines
- VernacularTitle:人S100A6对人骨肉瘤细胞系?-catenin的作用
- Author:
Jia WEI
;
Yinghua CHEN
;
Limei WU
;
Wen MA
;
Yan ZHANG
;
Tongchuan HE
;
Lan ZHOU
- Publication Type:Journal Article
- Keywords:
S100A6;
?-catenin;
osteosarcoma
- From:
Basic & Clinical Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of human S100A6 on ?-catenin in human osteosarcoma cell lines MG63 and U2OS.Methods Cell lines MG63 and U2OS were infected by recombinant adenoviruses carrying human S100A6 and its siRNA gene,AdS100A6 and AdSiS100A6 respectively,to up-regulate and down-regulate the expression of S100A6. Then RT-PCR,Western blot and immunocytochemistry were used to detect mRNA and protein (level and/or distribution) of ?-catenin.Results In both cell lines,with up-regulated S100A6,expression of ?-catenin mRNA and protein increased(P
