Effects of aspirin on [Ca~(2+)]i and NGB in cultured neural cells of newborn rat under chemical-induced hypoxic exposure
- VernacularTitle:阿司匹林对化学性缺氧状态下新生大鼠脑神经细胞[Ca~(2+)]i和NGB的影响
- Author:
Ju CHENG
;
Decheng BAI
;
Kun XIE
;
Min HOU
- Publication Type:Journal Article
- Keywords:
aspirin;
primary cell culture;
hypoxia;
intracellular free calcium concentration;
neuroglobin
- From:
Basic & Clinical Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of aspirin on hypoxic brain neural cells of rat and the effects of extracellular calcium on the neuroprotective effect of aspirin. Methods The hypoxic cell model was established by adding Na2S2O4 to the calcium or calcium free medium. Cultured primary cortical neurons of rat were pretreated with ASA in vitro. The change of intracellular free calcium and neuroglobin (NGB) were observed and analyzed by laser scanning confocal microscope. Results The expression level of [Ca2+]i and NGB increased significantly in the chemical hypoxia group(P
