Construction of eukaryotic expression vector containing telomere/telomerase binding factor PinX1 and its expression in Hek293 cells
- VernacularTitle:PinX1基因真核表达载体的构建及其在Hek293细胞中的表达
- Author:
Wendan SUN
- Publication Type:Journal Article
- Keywords:
PinXl;
RT-PCR;
immunocytochemical methods
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the cellular distribution of PinX1 protein in transfected Hek293 cells.Methods The PinX1 mRNA was amplified from HepG2 cells by RT-PCR and inserted into pcDNA3-vsv vector,and the vector was transfected into Hek293 cells. The expressed protein was detected by immunocytochemical method.Results PinX1 mRNA from HepG2 was obtained and the recombinant vector pcDNA3-PinX1-vsv was successfully constructed.Immunocytochemical method verified that PinX1 was expressed in nuclei after transfected.Conclusion We successfully got PinX1 mRNA,and it could be expressed in nuclei of Hek293 cells.This sets up a solid foundation for PinX1's function study of mediating telomere and telomerase.
