The localization of histidine triad protein of Streptococcus suis 2
- VernacularTitle:组氨酸三聚体蛋白在2型猪链球菌的定位
- Author:
Zhuqing SHAO
- Publication Type:Journal Article
- Keywords:
histidine triad protein;
flow cytometry;
surface localization;
sortase A
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the localization of histidine triad protein(Htp) on Streptococcus suis 2(S.suis 2) and the effect of disruption of sortase A(srtA) gene on the expression level of Htp.Methods Antiserum against Htp was prepared by immunizing rabbit with recombinant Htp.Bioinformatics analysis was adopted to evaluate the amino acid sequence characteristic of Htp.Flow cytometry was applied to localize Htp on S.suis 2 bacteria.Results Bioinformatics analysis demonstrated that Htp had the characteristic of cell surface protein with signal sequence and transmembrane sequence.Flow cytometry showed the localization of Htp was on the surface of S.suis 2 and the expression level of Htp on cell surface decreased by deletion of srtA.Conclusions Htp is located on the cell surface of S.suis 2.The results provide a foundation for further investigation of the role of Htp in the infection of S.suis 2.
