Amplification of?-globin gene to assess the quality of genitourinary tract samples for PCR detection
- VernacularTitle:用?-珠蛋白基因监测泌尿生殖道标本PCR检测质量
- Author:
Jiansen CHEN
;
Zhixin CHEN
;
Wenjuan ZHOU
;
Bin ZHU
- Publication Type:Journal Article
- Keywords:
polymerase chain reaction;
Chlamydia trachomatis;
?-globin gene;
quality control of samples
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether amplification of?-globin gene can be used to monitor the quality of samples for PCR detection of DNA of Chlamydia trachomatis(CT).Methods First-void urines(FVU),endocervical swabs(ECS),and urethral swabs (UTS) were collected for PCR detection of CT-DNA and cellular?-globin gene.Samples negative for?-globin gene were retested after 10-fold dilution.Results The positive rates of?-globin gene in FVU and ECS were 95.6%(255/264),and 91.7%(413/450) respectively, both higher than that(77.8%,172/221) in UTS(P
