Detection of serum protein biomarkers by surface enhanced laser desorption ionization in patients with lung adenocarcinoma
- VernacularTitle:用SELDI-TOF-MS技术筛查肺腺癌血清蛋白质标志物
- Author:
Min JIANG
;
Guohao GU
;
Wenjing WANG
;
Ping LIAO
;
Heng HU
- Publication Type:Journal Article
- Keywords:
adenocarcinoma of lung;
SELDI-TOF-MS;
diagnosis
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify new serum biomarkers of lung adenocarcinoma.Methods Serum samples from 31 patients with lung adenocarcinoma and 31 healthy individuals were applied to SAX-2 protein chips to generate proteomic spectra by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS).The spectra were analyzed with Ciphergen Biosystems software and biomarker patterns software.Results The software identified 102 peaks and m/z 14 022.9 and 3 735.99 were used to construct the classification tree.The classification tree separated adenocarcinoma of lung effectively from healthy individuals,achieving a validity of 100%.The blind test challenged the model with a sensitivity of 100%and a specificity of 100%.Conclusions The results suggest that SELDI-TOF-MS technique can distinguish lung adenocarcinoma patients from healthy individuals and shows great potential for the development of a screening test for the detection of lung cancer.
