Detecting of Class D Carbapenemase Gene in Acinetobacter baumannii by Multiplex PCR
- VernacularTitle:多重PCR快速检测鲍氏不动杆菌D类碳青酶烯酶相关基因
- Author:
Zheng WANG
;
Ping CHEN
;
Ding LIU
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
Carbapenemase;
Multiplex PCR
- From:
Chinese Journal of Nosocomiology
2009;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a rapid method of detecting class D carbapenemase gene in Acinetobacter baumannii by multiplex PCR.METHODS Design primers according to the conservative region of blaOXA-51 and blaOXA-23,the class D carbapenemase gene were detected by Multiplex PCR.RESULTS 24 of 30 A.baumannii isolates produced two bands of blaOXA-51 and blaOXA-23;other 6 isolates showed a band of blaOXA-23.CONCLUSIONS The multiplex PCR could detect rapidly the class D carbapenemase gene.
