Content Mensuration of Dehydroandrographolide in Ganmaoqing Capsule by HPLC
10.3760/cma.j.issn.1008-6706.2010.23.020
- VernacularTitle:感冒清胶囊中脱水穿心莲内酯的测定方法
- Author:
Zhigang SONG
;
Qian DING
- Publication Type:Journal Article
- Keywords:
Ganmaoqing;
Dehydroandrographolide
- From:
Chinese Journal of Primary Medicine and Pharmacy
2010;17(23):3206-3207,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the determination of Dehydroandrographolide in Ganmaoqing Capsule. MethodsThe determination was carried out by HPLC with a KromasilC-18 column(250 mm ×4.6 mm,5μm),methanol-water(65:35)severed as the mobile phase,the speed was 1mL/min and the detection wavelength was at 254 nm. ResultsDehydroandrographolide showed a good linear relationship at the range of 0.04872 μg ～0.38976 μg(r2 =0.9995,n= 8);The average recovery of Dehydroandrographolide was 100.1%(RSD= 2.92%). ConclusionHPLC method was sensitive,accurate,reproducible,specific and could be used for quality control of Ganmaoqing Capsule efficiently.
