The Liver X Receptor Ligand Agonist Inhibits Hypertrophy in Murine HL-1 Cardiomyocytes in vitro
- VernacularTitle:肝X受体激动剂抑制HL-1心肌细胞的肥大
- Author:
Jiang LI
;
Wenjuan GU
;
Sai NIE
;
Daoquan PENG
;
Shuiping ZHAO
- Publication Type:Journal Article
- Keywords:
Liver X receptors;
Agonist;
Cardiomyocyte hypertrophy;
Angiotensin Ⅱ
- From:
Chinese Journal of Hypertension
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Background Liver X receptors (LXRs) are members of the nuclear hormone receptor superfamily which involve in energy metabolism of intracellular cholesterol and glucose regulation.Objective To investigate the effect of Liver X receptors (LXRs) agonists on the hypertrophy induced by angiotensin Ⅱ(Ang Ⅱ) in the murine HL-1 cardiomyocytes in vitro.Methods Hypertrophy in murine HL-1 cardiomyocytes was induced by Ang Ⅱ and treated with LXRs agonist T0901317 (1 ?mol/L).Immunofluorescent staining was carried out to identify the HL-1 cells.The surface area of HL-1 cells was analyzed by using NIH Image J software.The synthetic rate of protein in HL-1 cells was detected by {}3H leucine incorporation.The mRNA level of atrial natriuretic peptide (ANP) was measured by quantitative realtime PCR.Results HL-1 cells hypertrophy induced by Ang Ⅱ were manifested by the increases in surface area,mRNA expression of ANP,and {}3H leucine incorporation(control group vs Ang Ⅱ group:cell surface:1.00?0.16 vs 2.00?0.21,ANP mRNA:1.00?0.02 vs 1.58?0.27,{}3H leucine incorporation:1.00?0.03 vs 1.44?0.07,respectively,all P
