Aminoguanidine Improved the Angiogenesis Induced by Tissue Ischemia in Diabetic Mice
- VernacularTitle:氨基胍改善糖尿病小鼠组织缺血后血管新生障碍
- Author:
Ying LIU
;
Li DONG
;
Lina KANG
;
Biao XU
- Publication Type:Journal Article
- Keywords:
Aminoguanidine;
Diabetes mellitus;
Limb ischemia;
Angiogenesis;
Advanced glycation end products
- From:
Chinese Journal of Hypertension
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Using a mouse hind limb ischemia model,we compared the ischemia-induced angiogenesis between diabetic and non-diabetic mice,and the effects aminoguanidine,an inhibitor of advanced glycation end products (AGEs),on angiogenesis in diabetic mice.Methods Experimental diabetic models were induced by injected with streptozotocin (STZ)[40 mg/(kg?d),Ip] in C57BL/6 mice.Two weeks after the establishment of diabetic model,diabetic mice were randomly to receive placebo(n=61)or aminoguanidine[200 mg/(kg?d) gavage,n=61) for 8 weeks.Tissue ischemia was obtained by ligation of left femoral artery.Capillary density was determined in the ischemic and non-ischemic limbs by CD31 staining at days 0,1,3,7,14 and 28 post-operatively.Results Compared with non-diabetic controls,diabetic mice had reduced microvascular density[(6.92?0.85) vs (9.21?1.64)/HP,P
