Expression of Luteinizing Hormone (LH) Gene in Rat Uterus and Epididymis.
- Author:
Sung Ho LEE
;
Youngki LEE
- Publication Type:Original Article
- MeSH:
Animals;
Complex Mixtures;
DNA, Complementary;
Epididymis*;
Exons;
Female;
Lutein*;
Luteinizing Hormone*;
Male;
Ovary;
Radioimmunoassay;
Rats*;
Testis;
Tissue Extracts;
Uterus*
- From:Korean Journal of Fertility and Sterility
1999;26(2):157-161
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Recent studies clearly demonstrated that the novel expression of LH gene in the rat testis, and suggested the local action of the LH-like molecule. The present study was performed to analyze the expression of the LH genes in the rat accessory reproductive organs. Expression of LH subunit genes in the rat uterus and epididymis was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) and specific LH radioimmunoassay (RIA). The LHbeta transcripts in these organs contained the published cDNA structure, the pituitary type exons 1-3, which encoded the entire LHbeta/ polypeptide. Presence of the transcripts for the alpha-subunit in the rat reproductive tissues were also confirmed by RT-PCR. In the LH RIA, significant levels of LH were detected in crude extracts from the rat ovary, uterus and epididymis. The competition cuties with increasing amount of tissue extracts were parallel with those of standard peptide, indicating that the immunoreactive LH-like materials in these tissues are similar to authentic pituitary LH molecule. In rat epididymis, the highest amount of immunoreactive LH was detected in corpus area. Our findings demonstrated that the genes for LH subunits are expressed in the rat accessory reproductive organs, and suggested that these extrapituitary LH may act as a local regulator with auto and/or paracrine manner.
