TrKA-siRNA inhibits the expression of NF-?B and promote apoptosis of breast cancer cell line MCF-7
- VernacularTitle:TrKA-siRNA抑制乳腺癌细胞系MCF-7 NF-?B的表达并促细胞凋亡
- Author:
Ju ZHANG
;
Changjie CHEN
;
Chenbiao LIU
;
Qingling YANG
;
Fengmeng TENG
- Publication Type:Journal Article
- Keywords:
small interfering RNA;
NF-?B;
TrKA;
breast tumor;
MCF-7 cell
- From:
Basic & Clinical Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Investigate the effect of TrKA variation on the expression of neucleoprotein NF-?B P65 and apoptosis.Methods To construct the expression vector of TrKA small interfering RNA,the recombinant was transfected into MCF-7 cells.the stable cell line expressing TrKA small interfering RNA were selected by G418.The mRNA and protein of TrKA were tested by real-time PCR,Western-blot and Immunohistochemistry.The change of neucleoprotein NF-?B P65 was detected by WB,Flow cytometry was used to observe the cell apoptosis.ResultsThe expression vector of TrKA-siRNA was successfully constructed.The mRNA and protein of TrKA were decreased by 74.7% and 80.5% respectively(P
